|Application ||WB, IHC, E|
|Preparation||Produced from sera of goats pre-immunized with highly pure recombinant Human SDF-1β. Anti-Human SDF-1β specific antibody was purified by affinity chromatography employing immobilized Human SDF-1β matrix.|
|WesternBlot||To detect Human SDF-1β by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.|
|Sandwich||To detect Human SDF-1β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human SDF-1β (60-295BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF-1β.|
|Immunohistochemistry||This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas malignant adenocarcinoma. The recommended concentration is 2.5-5.0 µg/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 9.0 buffer is recommended. Optimal concentrations and conditions may vary. |
Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
Information and photo are courtesy of Flagship Biosciences, LLC – Histology Services.
|Formulation||A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Human SDF-1β Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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