|Application ||WB, IHC-P|
|Description||Rabbit IgG polyclonal antibody for cAMP-dependent protein kinase type I-alpha regulatory subunit(PRKAR1A) detection. Tested with WB, IHC-P in Human;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||cAMP-dependent protein kinase type I-alpha regulatory subunit, Tissue-specific extinguisher 1, TSE1, cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed, PRKAR1A, PKR1, PRKAR1, TSE1|
|Calculated MW||42982 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, By Heat|
Western blot, 0.1-0.5 µg/ml, Human, Rat
|Subcellular Localization||Cell membrane .|
|Tissue Specificity||Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.|
|Protein Name||cAMP-dependent protein kinase type I-alpha regulatory subunit|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E.coli-derived human PRKAR1A recombinant protein (Position: E2-E81). Human PRKAR1A shares 89.9% and 92.4% amino acid (aa) sequence identity with mouse and rat PRKAR1A, respectively.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Synonyms||PKR1, PRKAR1, TSE1|
|Function||Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.|
|Cellular Location||Cell membrane.|
|Tissue Location||Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible|
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Provided below are standard protocols that you may find useful for product applications.
cAMP-dependent protein kinase type I-alpha regulatory subunit is an enzyme that in humans is encoded by the PRKAR1A gene. This protein encoded by this gene was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed.
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