|Description||Rabbit IgG polyclonal antibody for Lymphocyte antigen 6A-2/6E-1(Ly6a) detection. Tested with WB in Mouse.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Lymphocyte antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Stem cell antigen 1, SCA-1, T-cell-activating protein, TAP, Ly6a, Ly6|
|Calculated MW||14377 MW KDa|
|Application Details||Western blot, 0.1-0.5 µg/ml, Mouse|
|Subcellular Localization||Cell membrane; Lipid-anchor, GPI-anchor.|
|Tissue Specificity||Widely expressed. .|
|Protein Name||Lymphocyte antigen 6A-2/6E-1|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of mouse Sca1/Ly6A/E (48-75aa YPDGVCVTQEAAVIVDSQTRKVKNNLCL).|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins.|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor.|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Stem cell antigen-1 (Sca-1) is a mouse glycosyl phosphatidylinositol-anchored protein and a cell surface marker found on hematopoietic stem cells (HSCs). It is encoded by the strain-specific Ly-6 E/A allelic gene. Sca-1 has been discovered in several non hematopoietic tissues and can be used to enrich progenitor cell populations other than HSC. It is suggested that Sca-1 could be involved in regulating both B and T cell activation.
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