|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat|
|Description||Mouse IgG monoclonal antibody for Calbindin-D, calbindin 1, 28kDa (CALB1) detection. Tested with WB, IHC-P in Human;mouse;rat;bovine. No cross reactivity with other proteins.|
|Reconstitution||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Other Names||Calbindin, Calbindin D28, D-28K, Spot 35 protein, Vitamin D-dependent calcium-binding protein, avian-type, Calb1|
|Calculated MW||29994 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 1-2 µg/ml, Human, bovine, mouse, rat, By Heat|
Western blot, 0.5-1 µg/ml, Human, bovine, mouse, rat
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Bovine kidney calbindin-D|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the calbindin family.|
|Function||Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.|
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Provided below are standard protocols that you may find useful for product applications.
The human calbindin D28k(CALB1) is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. Parmentier et al.(1991) mapped the CALB1 gene to 8q21.3-q22.1 by in situ hybridization.
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