|Application ||WB, IHC-P|
|Description||Mouse IgG monoclonal antibody for Laminin, laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2 (laminin S) (LAMA1; LAMA2; LAMB1; LAMB2) detection. Tested with WB, IHC-P in Human;feline;pig. No cross reactivity with other proteins.|
|Reconstitution||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Other Names||Laminin subunit beta-2, Laminin chain B3, Laminin-11 subunit beta, Laminin-14 subunit beta, Laminin-15 subunit beta, Laminin-3 subunit beta, Laminin-4 subunit beta, Laminin-7 subunit beta, Laminin-9 subunit beta, S-laminin subunit beta, S-LAM beta, Lamb2|
|Calculated MW||196474 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, feline, pig, By Heat|
Western blot, 0.25-0.5 µg/ml, Human, feline, pig
|Subcellular Localization||Secreted, extracellular space, extracellular matrix, basement membrane.|
|Tissue Specificity||Found in the basement membranes (major component). S-laminin is concentrated in the synaptic cleft of the neuromuscular junction.|
|Protein Name||Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Contains 17 laminin EGF-like domains.|
|Function||Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.|
|Cellular Location||Secreted, extracellular space, extracellular matrix, basement membrane|
|Tissue Location||Found in the basement membranes (major component). S-laminin is concentrated in the synaptic cleft of the neuromuscular junction|
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Provided below are standard protocols that you may find useful for product applications.
Laminin is a heterotrimeric extracellular matrix protein consisting of 3 chains: alpha-1,beta-1 and gamma-1, formerly called beta-2(LAMA2). This gene is over 260,000 base pairs and contains 64 exons. Laminin is similar with merosin, a basement membrane-associated protein found in placenta, striated muscle, and peripheral nerve, and both of them are members of the same family of basement membrane proteins. And merosin is the same as laminin M, a striated muscle-specific, basal-lamina-associated protein, it may play a primary role in the pathogenesis of that disorder.
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