|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat|
|Description||Mouse IgG monoclonal antibody for beta-Tubulin detection. Tested with WB, IHC-P in Human,mouse,rat. No cross reactivity with other proteins.|
|Reconstitution||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Other Names||Tubulin beta-1 chain, TUBB1|
|Calculated MW||50327 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, mouse, rat, By Heat|
Western blot, 2 µg/ml, Human, mouse, rat
|Subcellular Localization||Cytoplasm, cytoskeleton .|
|Tissue Specificity||Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins. .|
|Protein Name||Tubulin beta-1 chain|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Clone Names||TUB 2.1|
|Immunogen||Tubulin from rat brain.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Function||Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).|
|Cellular Location||Cytoplasm, cytoskeleton|
|Tissue Location||Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins|
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Provided below are standard protocols that you may find useful for product applications.
Tubulin beta(TUBB) was derived from its corresponding mRNA with a molecular weight of about 55,000. TUBB gene contains 4 exons and located in the HLA class I region at 6p21.3. Paclitaxel resistance in non-small-cell lung cancer associated with beta-tubulin gene mutations.
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