|Application ||WB, IHC-P, IHC-F, ICC|
|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for Lamin-B1(LMNB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Lamin-B1, LMNB1, LMN2, LMNB|
|Calculated MW||66408 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, By Heat|
Immunocytochemistry , 0.5-1 µg/ml, Human, Rat
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Rat, Human
Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
|Subcellular Localization||Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B1(570-586aa FHQQGTPRASNRSCAIM), different from the related rat sequence by one amino acid, and from the related mouse sequence by three amino acids.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the intermediate filament family.|
|Function||Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.|
|Cellular Location||Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side|
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Provided below are standard protocols that you may find useful for product applications.
Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are though to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.
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