|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for Acetylcholine receptor subunit alpha(CHRNA1) detection. Tested with WB in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Acetylcholine receptor subunit alpha, CHRNA1, ACHRA, CHNRA|
|Calculated MW||54546 MW KDa|
|Application Details||Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse|
|Subcellular Localization||Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.|
|Tissue Specificity||Isoform 1 is only expressed in skeletal muscle. Isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus.|
|Protein Name||Acetylcholine receptor subunit alpha|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Nicotinic Acetylcholine Receptor alpha 1(22-36aa EHETRLVAKLFKDYS), identical to the related rat sequence, different from the related mouse sequence by one amino acid.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Function||After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.|
|Cellular Location||Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein|
|Tissue Location||Isoform 1 is only expressed in skeletal muscle. Isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus|
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Provided below are standard protocols that you may find useful for product applications.
CHRNA, also termed ACHRA, is mapped on 2q24-q32.This gene encodes the alpha subunit of the muscle acetylcholine receptor, which is the main target of pathogenic autoantibodies in autoimmune myasthenia gravis. The protein-coding sequence of the human alpha subu gene is divided into 9 exons that correspond to different structural and functional domains of the precursor molecule.
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