|Application ||WB, IHC-P, IHC-F|
|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for Podocin(NPHS2) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Podocin, NPHS2|
|Calculated MW||42201 MW KDa|
|Application Details||Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Rat, Human, Mouse|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Rat, Human, Mouse
|Subcellular Localization||Isoform 1: Cell membrane ; Peripheral membrane protein .|
|Tissue Specificity||Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human NPHS2(368-383aa KPVEPLNPKKKDSPML), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the band 7/mec-2 family.|
|Function||Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.|
|Cellular Location||Isoform 1: Cell membrane; Peripheral membrane protein|
|Tissue Location||Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli|
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Provided below are standard protocols that you may find useful for product applications.
Podocin(PDCN) is a protein which lines the podocytes and assists in maintaining the barrier at the glomerular basement membrane. NPHS2 is a causative gene for Familial idiopathic nephrotic syndromes, which represents a heterogeneous group of kidney disorders, and include autosomal recessive steroid-resistant nephrotic syndrome, which is characterized by early childhood onset of proteinuria, rapid progression to end-stage renal disease and focal segmental glomerulosclerosis. By positional cloning, NPHS2 was mapped to 1q25-31. It is almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli, and encodes a new integral membrane protein, podocin, belonging to the stomatin protein family. Ten different NPHS2 mutations were found, comprising nonsense, frameshift and missense mutations, to segregate with the disease, demonstrating a crucial role for podocin in the function of the glomerular filtration barrier.
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