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Anti-SNAP23 Antibody

  • WB - Anti-SNAP23 Antibody ABO11081
    Anti-SNAP23 antibody, ABO11081, Western blottingLane 1: Rat Spleen Tissue LysateLane 2: Rat testis Tissue LysateLane 3: Rat Ovary Tissue LysateLane 4: HELA Cell LysateLane 5: MCF-7 Cell LysateLane 6: SKOV Cell Lysate
  • IHC - Anti-SNAP23 Antibody ABO11081
    Anti-SNAP23 antibody, ABO11081, IHC(P)IHC(P): Human Placenta Tissue
  • IHC - Anti-SNAP23 Antibody ABO11081
    Anti-SNAP23 antibody, ABO11081, IHC(F)IHC(F): Human Placenta Tissue
  • ICC - Anti-SNAP23 Antibody ABO11081
    Anti-SNAP23 antibody, ABO11081,ICCICC: K562 Cell
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00161
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Synaptosomal-associated protein 23(SNAP23) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 8773
Other Names Synaptosomal-associated protein 23, SNAP-23, Vesicle-membrane fusion protein SNAP-23, SNAP23
Calculated MW 23354 MW KDa
Application Details Immunocytochemistry , 0.5-1 µg/ml, Human, -
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse
Subcellular Localization Cell membrane; Peripheral membrane protein. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.
Tissue Specificity Ubiquitous. Highest levels where found in placenta.
Protein Name Synaptosomal-associated protein 23(SNAP-23)
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP23(192-211aa DTNRDRIDIANARAKKLIDS), different from the related rat and mouse sequences by three amino acids.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the SNAP-25 family.
Protein Information
Name SNAP23
Function Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.
Cellular Location Cell membrane; Peripheral membrane protein. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome Note=Mainly localized to the plasma membrane
Tissue Location Ubiquitous. Highest levels where found in placenta
Research Areas
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SNAP23(Synaptosomal-Associated Protein, 23-KD), also called SNAP23A, is a protein that in humans is encoded by the SNAP23 gene. The SNAP23 gene has 8 exons, with the initiation codon located in exon 2. The SNAP23 gene is mapped on 15q15.1-q15.2. The SNAP23 cDNA encodes a 211-amino acid polypeptide with a predicted mass of 23 kD. Its amino acid sequence is 59% identical to that of SNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. SNAP23 is able to bind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After relocation, SNAP23 is required for exocytosis, implying a crucial role in promoting membrane fusion. TIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A and SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl maleimide-dependent SNARE complexes with TIVAMP and cellubrevin. SNAP23 is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion.

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$ 280.00
Cat# ABO11081
Availability: 3-5 days
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