|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for DNA-3-methyladenine glycosylase(MPG) detection. Tested with WB in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||DNA-3-methyladenine glycosylase, 220.127.116.11, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, ADPG, N-methylpurine-DNA glycosylase, MPG, AAG, ANPG, MID1|
|Calculated MW||32869 MW KDa|
|Application Details||Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse|
|Subcellular Localization||Cytoplasm . Mitochondrion matrix, mitochondrion nucleoid . Nucleus .|
|Protein Name||DNA-3-methyladenine glycosylase|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MPG(228-243aa NKSFDQRDLAQDEAVW), different from the related mouse sequence by two amino acids, and from related rat sequence by one amino acid.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the DNA glycosylase MPG family.|
|Synonyms||AAG, ANPG, MID1|
|Function||Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.|
|Cellular Location||Cytoplasm. Mitochondrion matrix, mitochondrion nucleoid. Nucleus|
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Provided below are standard protocols that you may find useful for product applications.
MPG(N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG or APNG. The MPG gene is mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines.Â TheÂ MPGÂ gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line(HT29). The completely characterized humanÂ MPGÂ gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo,Â Hang et al.(1997)Â biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine(meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.
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