|Description||Rabbit IgG polyclonal antibody for Neurogenic locus notch homolog protein 4(NOTCH4) detection. Tested with WB in Human.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Neurogenic locus notch homolog protein 4, Notch 4, hNotch4, Notch 4 extracellular truncation, Notch 4 intracellular domain, NOTCH4, INT3|
|Calculated MW||209622 MW KDa|
|Application Details||Western blot, 0.1-0.5 µg/ml, Human|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein.|
|Tissue Specificity||Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.|
|Protein Name||Neurogenic locus notch homolog protein 4(Notch 4)|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human NOTCH4(1805-1818aa DVAHQRNHWDLLTL).|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the NOTCH family.|
|Function||Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity).|
|Cellular Location||Cell membrane; Single-pass type I membrane protein|
|Tissue Location||Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes|
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NOTCH4(NOTCH, DROSOPHILA, HOMOLOG OF, 4) also known asÂ INT3 or NOTCH3, FORMERLY, is a member of the Notch family. In situ hybridization revealed thatÂ Notch4Â transcripts are primarily restricted to endothelial cells in embryonic and adult life, suggesting a specific role forNotch4Â during development of vertebrate endothelium. The sequences of the mouse and humanÂ NOTCH4Â proteins are 82% identical.Â Northern blot analysis revealed thatÂ NOTCH4(S) is the major transcript and is expressed in a wide variety of tissues. Fluorescence in situ hybridization confirmed the location of theÂ NOTCH4Â gene at chromosome 6p21.3. In linkage disequilibrium mapping of the MHC region in 80 British parent-offspring trios,Â Wei and Hemmings(2000)Â found thatÂ NOTCH4Â was highly associated with schizophrenia. Repression ofÂ Notch4Â resolved ataxia and reversed the disease progression, demonstrating thatÂ Notch4Â is not only sufficient to induce but also required to sustain the disease.Â
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