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Anti-TJP2 Antibody

  • WB - Anti-TJP2 Antibody ABO11279
    Anti-TJP2 antibody, ABO11279, Western blottingLane 1: Rat Brain Tissue LysateLane 2: PC-12 Cell LysateLane 3: Mouse Brain Tissue LysateLane 4: HEPA Cell Lysate
  • IHC - Anti-TJP2 Antibody ABO11279
    Anti-TJP2 antibody, ABO11279, IHC(P)IHC(P): Mouse Liver Tissue
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Z0U1
Host Rabbit
Reactivity Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Tight junction protein ZO-2(TJP2) detection. Tested with WB, IHC-P in Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 21873
Other Names Tight junction protein ZO-2, Tight junction protein 2, Zona occludens protein 2, Zonula occludens protein 2, Tjp2, Zo2
Calculated MW 131280 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Mouse, Rat
Subcellular Localization Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Nucleus . Cell junction, tight junction . Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures. .
Protein Name Tight junction protein ZO-2
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse TJP2(1149-1167aa AHSKRGYYSQPSRYRDTEL), identical to the related rat sequence.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the MAGUK family.
Protein Information
Name Tjp2
Synonyms Zo2
Function Plays a role in tight junctions and adherens junctions.
Cellular Location Cell membrane; Peripheral membrane protein; Cytoplasmic side Nucleus. Cell junction, tight junction. Note=Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures.
Research Areas
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TJP2(Tight Junction Protein 2), also known as Zona Occludens 2 or ZO2, is a protein that in humans is encoded by the TJP2 gene. Tight junction proteins(TJPs) belong to a family of membrane-associated guanylate kinase(MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. Duclos et al.(1994) mapped the TJP2 gene telomeric to the Friedreich ataxia critical region on chromosome 9q13-q21. TJP2 lies about 70 kb centromeric to the X123 gene and is transcribed in the centromere-to-telomere direction. Using in vitro assays and immunoprecipitation studies, Itoh et al.(1999) showed that the mouse Tjp1, Tjp2, and Tjp3 PDZ1 domains interacted with the C-terminal cytoplasmic domains of Cldn1 through Cldn8. In the mouse inner ear, Walsh et al.(2010) found that Tjp2 expression decreased rapidly between E16.5 and age 1 week to a level in adult mice that was approximately 50% of the level at birth(P0).

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$ 280.00
Cat# ABO11279
Availability: 3-5 days
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