|Description||Rabbit IgG polyclonal antibody for Inhibitor of nuclear factor kappa-B kinase subunit beta(IKBKB) detection. Tested with WB in Human.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, 220.127.116.11, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, IKBKB, IKKB|
|Calculated MW||86564 MW KDa|
|Application Details||Western blot, 0.1-0.5 µg/ml, Human|
|Subcellular Localization||Cytoplasm. Nucleus. Membrane raft. Colocalized with DPP4 in membrane rafts.|
|Tissue Specificity||Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.|
|Protein Name||Inhibitor of nuclear factor kappa-B kinase subunit beta|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human IKK beta(735-750aa TALDWSWLQTEEEEHS).|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.|
|Function||Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF- dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.|
|Cellular Location||Cytoplasm. Nucleus. Membrane raft. Note=Colocalized with DPP4 in membrane rafts.|
|Tissue Location||Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood|
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Provided below are standard protocols that you may find useful for product applications.
IKBKB(Inhibitor of Kappa Light Chain Gene Enhancer in B Cells, Kinase of, Beta), also known as IKKB or NFKBIKB , is a protein that in humans is encoded by the IKBKB gene. Shindo et al.(1998) mapped the IKBKB gene to chromosome 8p12-p11 by FISH. Mercurio et al.(1997) found that mutations in IKK2 had a more pronounced effect upon NFKB activation than did comparable mutations in IKK1. Yin et al.(1998) tested the activity of various antiinflammatory agents on the IKK complex. They demonstrated that aspirin and sodium salicylate specifically inhibit IKK-beta activity in vitro and in vivo by binding to IKK-beta to reduce ATP binding.
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