|Application ||WB, IHC-P|
|Description||Rabbit IgG polyclonal antibody for Cystatin-A(CSTA) detection. Tested with WB, IHC-P in Human.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Cystatin-A, Cystatin-AS, Stefin-A, Cystatin-A, N-terminally processed, CSTA, STF1, STFA|
|Calculated MW||11006 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat|
Western blot, 0.1-0.5 µg/ml, Human
|Subcellular Localization||Cytoplasm .|
|Tissue Specificity||Expressed in the skin throughout the epidermis. .|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Cystatin A(77-90aa NEDLVLTGYQVDKN).|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the cystatin family.|
|Function||This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.|
|Tissue Location||Expressed in the skin throughout the epidermis.|
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Provided below are standard protocols that you may find useful for product applications.
Cystatin A also called Stefin A is a protein that in humans is encoded by the CSTA gene. Cystatin A is a cysteine proteinase inhibitor that belongs to family 1 of the cystatin superpamily. This gene was assigned to human chromosome 3q21.1. This is an intracellular thiol proteinase inhibitor. Cystatin A plays an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis. Stefins have been proposed as prognostic and diagnostic tools for cancer.
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