|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for Induced myeloid leukemia cell differentiation protein Mcl-1(MCL1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl2-L-3, Bcl-2-related protein EAT/mcl1, mcl1/EAT, MCL1, BCL2L3|
|Calculated MW||37337 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat|
Western blot, 0.1-0.5 µg/ml, Human, Rat
|Subcellular Localization||Membrane ; Single-pass membrane protein . Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Cytoplasmic, associated with mitochondria.|
|Protein Name||Induced myeloid leukemia cell differentiation protein Mcl-1|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E.coli-derived human MCL1 recombinant protein (Position: M1-R350). Human MCL1 shares 82% and 78% amino acid (aa) sequences identity with mouse and rat MCL1, respectively.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the Bcl-2 family.|
|Function||Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.|
|Cellular Location||Membrane; Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Note=Cytoplasmic, associated with mitochondria|
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Provided below are standard protocols that you may find useful for product applications.
MCL1, also known as myeloid cell leukemia 1, is a protein that in humans is encoded the MCL1 gene. It is mapped to 1q21.3. MCL1 is a potent multidomain antiapoptotic protein of the BCL2 family that heterodimerizes with other BCL2 family members to protect against apoptotic cell death. MCL1 as an attractive candidate for regulation of hematopoietic stem cell homeostasis that is highly expressed in hematopoietic stem cells and regulated by growth factor signals. MCL1 is a critical and specific regulator essential for ensuring the homeostasis of early hematopoietic progenitors. During mitotic arrest, MCL1 protein levels decline markedly, through a posttranslational mechanism, potentiating cell death. Phosphorylation of MCL1 directs its interaction with the tumor suppressor protein FBW7, which is the substrate-binding component of a ubiquitin ligase complex.
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