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Anti-SLC9A1 Picoband Antibody

  • WB - Anti-SLC9A1 Picoband Antibody ABO11849
    Anti-SLC9A1 Picoband antibody, ABO11849-1.jpgAll lanes: Anti SLC9A1 (ABO11849) at 0.5ug/mlWB: Recombinant Human Sodium Protein 0.5ngPredicted bind size: 37KDObserved bind size: 37KD
  •  - Anti-SLC9A1 Picoband Antibody ABO11849
    Anti-SLC9A1 Picoband antibody, ABO11849-2.jpgAll lanes: Anti SLC9A1 (ABO11849) at 0.5ug/mlLane 1: PC-12 Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 91KDObserved bind size: 91KD
  • IHC - Anti-SLC9A1 Picoband Antibody ABO11849
    Anti-SLC9A1 Picoband antibody, ABO11849-3.JPGIHC(P): Rat Kidney Tissue
  • IHC - Anti-SLC9A1 Picoband Antibody ABO11849
    Anti-SLC9A1 Picoband antibody, ABO11849-4.JPGIHC(P): Human Mammary Cancer Tissue
  • IHC - Anti-SLC9A1 Picoband Antibody ABO11849
    Anti-SLC9A1 Picoband antibody, ABO11849-5.JPGIHC(P): Mouse Kidney Tissue
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19634
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Sodium/hydrogen exchanger 1(SLC9A1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 6548
Other Names Sodium/hydrogen exchanger 1, APNH, Na(+)/H(+) antiporter, amiloride-sensitive, Na(+)/H(+) exchanger 1, NHE-1, Solute carrier family 9 member 1, SLC9A1, APNH1, NHE1
Calculated MW 90763 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Rat
Subcellular Localization Membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell membrane; Multi-pass membrane protein. Colocalizes with CHP1 at the reticulum endoplasmic (By similarity). Colocalizes with CHP1 and CHP2 at the plasma membrane. .
Tissue Specificity Kidney and intestine.
Protein Name Sodium/hydrogen exchanger 1
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human SLC9A1 recombinant protein (Position: H543-Q815). Human SLC9A1 shares 92% and 93% amino acid (aa) sequences identity with mouse and rat SLC9A1, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
Protein Information
Name SLC9A1
Synonyms APNH1, NHE1
Function Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
Cellular Location Membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein Note=Colocalizes with CHP1 at the reticulum endoplasmic (By similarity). Colocalizes with CHP1 and CHP2 at the plasma membrane.
Tissue Location Kidney and intestine.
Research Areas
Citations (0)

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NHE1, also known as SLC9A1, is an isoform of sodium–hydrogen antiporter that in humans is encoded by the SLC9A1 gene. It is mapped to 1p36.11. NHE1 is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others. The plasma membrane ion exchanger NHE1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. And a structural role for NHE1 in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. NHE1 is a plausible candidate gene for human essential hypertension.

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$ 280.00
Cat# ABO11849
Availability: 3-5 days
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