|Application ||WB, IHC-F, FC, ICC|
|Description||Rabbit IgG polyclonal antibody for T-lymphocyte activation antigen CD86(CD86) detection. Tested with WB, IHC-F, ICC, FCM in Human.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD86, CD86, CD28LG2|
|Calculated MW||37682 MW KDa|
|Application Details||Immunohistochemistry(Frozen Section), 0.5-1 µg/ml|
Immunocytochemistry, 0.5-1 µg/ml
Western blot, 0.1-0.5 µg/ml
Flow Cytometry, 1-3Î¼g/1x106cells
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein.|
|Tissue Specificity||Expressed by activated B-lymphocytes and monocytes.|
|Protein Name||T-lymphocyte activation antigen CD86|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E.coli-derived human CD86 recombinant protein (Position: A24-F329). Human CD86 shares 50% amino acid (aa) sequence identity with mouse CD86.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Contains 1 Ig-like C2-type (immunoglobulin-like) domain.|
|Function||Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein|
|Tissue Location||Expressed by activated B-lymphocytes and monocytes|
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Provided below are standard protocols that you may find useful for product applications.
Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.
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