|Application ||WB, IHC-P, IHC-F, ICC|
|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for Solute carrier family 2, facilitated glucose transporter member 1(SLC2A1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Solute carrier family 2, facilitated glucose transporter member 1, Glucose transporter type 1, erythrocyte/brain, GLUT-1, HepG2 glucose transporter, SLC2A1, GLUT1|
|Calculated MW||54084 MW KDa|
|Application Details||Immunocytochemistry , 0.5-1 µg/ml, Human, -|
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human
|Subcellular Localization||Cell membrane; Multi-pass membrane protein. Melanosome. Localizes primarily at the cell surface. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.|
|Tissue Specificity||Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues. .|
|Protein Name||Solute carrier family 2, facilitated glucose transporter member 1|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E.coli-derived human SLC2A1 recombinant protein (Position: R92-V492). Human SLC2A1 shares 98% and 98.3% amino acid (aa) sequence identity with mouse and rat SLC2A1, respectively.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.|
|Function||Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Melanosome. Note=Localizes primarily at the cell surface Identified by mass spectrometry in melanosome fractions from stage I to stage IV|
|Tissue Location||Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues|
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GLUT1, also known as SLC2A1, is a major glucose transporter in the mammalian blood-brain barrier whose gene is mapped to 1p35-p31.3 and contains 10 exons. It is present at high levels in primate erythrocytes and brain endothelial cells. Not only can transport dehydroascorbic acid (the oxidized form of vitamin C) into the brain, GLUT1 is also likely to contribute to HTLV-associated disorders through interacting with HTLV envelope glycoproteins. Functionally, GLUT1 deficiency causes a decrease in embryonic glucose uptake and apoptosis, which may be involved in diabetic embryopathy, by contrast, an increased expression of GLUT1 in some malignant tumors may suggest a role for glucose-derivative tracers to detect in vivo thyroid cancer metastases by positron-emission tomography scanning.
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