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Anti-CYP1A1 Picoband Antibody

     
  • WB - Anti-CYP1A1 Picoband Antibody ABO12231
    Anti- CYP1A1 Picoband antibody, ABO12231, Western blottingAll lanes: Anti CYP1A1 (ABO12231) at 0.5ug/mlLane 1: Rat Lung Tissue Lysate at 50ugLane 2: Mouse Lung Tissue Lysate at 50ugLane 3: Human Placenta Tissue Lysate at 50ugLane 4: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 58KDObserved bind size: 58KD
  • IHC - Anti-CYP1A1 Picoband Antibody ABO12231
    Anti- CYP1A1 Picoband antibody, ABO12231,IHC(P)IHC(P): Mouse Kidney Tissue
  • IHC - Anti-CYP1A1 Picoband Antibody ABO12231
    Anti- CYP1A1 Picoband antibody, ABO12231,IHC(P)IHC(P): Rat Kidney Tissue
  • IHC - Anti-CYP1A1 Picoband Antibody ABO12231
    Anti- CYP1A1 Picoband antibody, ABO12231,IHC(P)IHC(P): Human Mammary Cancer Tissue
  • IHC - Anti-CYP1A1 Picoband Antibody ABO12231
    Anti- CYP1A1 Picoband antibody, ABO12231,IHC(F)IHC(F): Mouse Kidney Tissue
  • IHC - Anti-CYP1A1 Picoband Antibody ABO12231
    Anti- CYP1A1 Picoband antibody, ABO12231,IHC(F)IHC(F): Human Placenta Tissue
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F
Primary Accession P04798
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Cytochrome P450 1A1(CYP1A1) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 1543
Other Names Cytochrome P450 1A1, 1.14.14.1, CYPIA1, Cytochrome P450 form 6, Cytochrome P450-C, Cytochrome P450-P1, CYP1A1
Calculated MW 58165 MW KDa
Application Details Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Human, Mouse, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue Specificity Lung, lymphocytes and placenta.
Protein Name Cytochrome P450 1A1
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human CYP1A1 recombinant protein (Position: H183-D320). Human CYP1A1 shares 81.2% amino acid (aa) sequence identity with both mouse and rat CYP1A1.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the cytochrome P450 family.
Protein Information
Name CYP1A1
Function Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Cellular Location Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P00185}; Peripheral membrane protein {ECO:0000250|UniProtKB:P00185}. Mitochondrion inner membrane {ECO:0000250|UniProtKB:P00185}; Peripheral membrane protein {ECO:0000250|UniProtKB:P00185}. Microsome membrane {ECO:0000250|UniProtKB:P00185}; Peripheral membrane protein {ECO:0000250|UniProtKB:P00185}. Cytoplasm {ECO:0000250|UniProtKB:P00185}
Tissue Location Lung, lymphocytes and placenta.
Research Areas
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Background

CYP1A1 is involved in phase I xenobiotic and drug metabolism (one substrate of it is theophylline). It is inhibited by fluoroquinolones and macrolides and induced by aromatic hydrocarbons. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH), for example, benzo(a)pyrene (BP), by transforming it to an epoxide. In this reaction, the oxidation of benzo[a]pyrene is catalysed by CYP1A1 to form BP-7,8-epoxide, which can be further oxidized by epoxide hydrolase (EH) to form BP-7,8-dihydrodiol. Finally CYP1A1 catalyses this intermediate to form BP-7,8-dihydrodiol-9,10-epoxide, which is the ultimate carcinogen. However, an in vivo experiment with gene-deficient mice has found that the hydroxylation of benzo(a)pyrene by CYP1A1 can have an overall protective effect on the DNA, rather than contributing to potentially carcinogenic DNA modifications. This effect is likely due to the fact that CYP1A1 is highly active in the intestinal mucosa, and thus inhibits infiltration of ingested benzo(a)pyrene carcinogen into the systemic circulation.

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$ 280.00
Cat# ABO12231
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