|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for Endothelin B receptor(EDNRB) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Endothelin B receptor, ET-B, ET-BR, Endothelin receptor non-selective type, EDNRB, ETRB|
|Calculated MW||49644 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat|
Western blot, 0.1-0.5 µg/ml, Human
|Subcellular Localization||Cell membrane; Multi-pass membrane protein.|
|Tissue Specificity||Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells. .|
|Protein Name||Endothelin B receptor|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human EDNRB (406-439aa QSFEEKQSLEEKQSCLKFKANDHGYDNFRSSNKY), different from the related mouse and rat sequences by one amino acid.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.|
|Function||Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Note=internalized after activation by endothelins.|
|Tissue Location||Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells|
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Endothelin receptor type B, also known as ETB is a protein that in humans is encoded by the EDNRB gene. The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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