|Application ||WB, IHC-P|
|Description||Rabbit IgG polyclonal antibody for Frizzled-1(FZD1) detection. Tested with WB, IHC-P in Human. |
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Frizzled-1, Fz-1, hFz1, FzE1, FZD1|
|Calculated MW||71158 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-2 µg/ml, Human, By Heat|
Western blot, 0.1-0.6 µg/ml, Human
|Subcellular Localization||Membrane; Multi-pass membrane protein. Cell membrane ; Multi-pass membrane protein .|
|Tissue Specificity||Expressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney.|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Frizzled homolog 1 (369-400aa YIAGFLLEDRVVCNDKFAEDGARTVAQG TKKE), identical to the related mouse and rat sequences.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the G-protein coupled receptor Fz/Smo family.|
|Function||Receptor for Wnt proteins (PubMed:10557084). Activated by WNT3A, WNT3, WNT1 and to a lesser extent WNT2, but apparently not by WNT4, WNT5A, WNT5B, WNT6, WNT7A or WNT7B (PubMed:10557084). Contradictory results showing activation by WNT7B have been described for mouse (By similarity). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:10557084). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (PubMed:10557084). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
|Tissue Location||Expressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
Frizzled-1 (Frizzled homolog 1) is a protein that in humans is encoded by the FZD1 gene. Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signalingproteins. The FZD1 protein contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. The FZD1 transcript is expressed in various tissues.
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