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Anti-HSPB2 Picoband Antibody

  • WB - Anti-HSPB2 Picoband Antibody ABO12284
    Anti-HSPB2 Picoband antibody, ABO12284, Western blottingAll lanes: Anti HSPB2 (ABO12284) at 0.5ug/mlLane 1: Rat Cardiac Muscle Tissue Lysate at 50ugLane 2: Rat Skeletal Muscle Tissue Lysate at 50ugPredicted bind size: 20KDObserved bind size: 20KD
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16082
Host Rabbit
Reactivity Human, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Heat shock protein beta-2(HSPB2) detection. Tested with WB in Human;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 3316
Other Names Heat shock protein beta-2, HspB2, DMPK-binding protein, MKBP, HSPB2
Calculated MW 20233 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Rat, Human
Subcellular Localization Cytoplasm . Nucleus . Localizes to nuclear foci.
Tissue Specificity Expressed preferentially in skeletal muscle and heart but not in the lens.
Protein Name Heat shock protein beta-2
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human HSPB2 recombinant protein (Position: M1-P182). Human HSPB2 shares 97.3% amino acid (aa) sequence identity with both mouse and rat HSPB2.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the small heat shock protein (HSP20) family.
Protein Information
Name HSPB2
Function May regulate the kinase DMPK.
Cellular Location Cytoplasm. Nucleus. Note=Localizes to nuclear foci
Tissue Location Expressed preferentially in skeletal muscle and heart but not in the lens
Research Areas
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Alpha-B-crystallin is a member of the alpha-crystallin/small heat-shock protein (HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al. (1997) identified a gene transcribed in the opposite direction. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They termed the gene HSPB2. Iwaki et al. (1997) also determined the complete genomic structure of the rat homolog of HSPB2. Southern blot analysis demonstrated that HSPB2 is expressed preferentially in skeletal muscle and heart but not in the lens, while the neighboring CRYAB gene is highly expressed in all 3 tissues. The 2 related genes are arranged in a head-to-head manner with an intergenic sequence of less than 1 kb, raising a possibility of shared regulatory elements for their expression.

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$ 240.00
Cat# ABO12284
Availability: 3-5 days
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