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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-SP-D Antibody   

Anti-SP-D Antibody

     
  • WB - Anti-SP-D Antibody ABO12303
    Anti- Surfactant protein D Picoband antibody, ABO12303, Western blottingAll lanes: Anti Surfactant protein D (ABO12303) at 0.5ug/mlLane 1: Rat Lung Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: PANC Whole Cell Lysate at 40ugPredicted bind size: 38KDObserved bind size: 38KD
  • IHC - Anti-SP-D Antibody ABO12303
    Anti- Surfactant protein D Picoband antibody, ABO12303, IHC(P)IHC(P): Human Lung Cancer Tissue
  • IHC - Anti-SP-D Antibody ABO12303
    Figure 3. IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (ABO12303).Surfactant protein D was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Surfactant protein D Antibody (ABO12303) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
  • IHC - Anti-SP-D Antibody ABO12303
    Figure 4. IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (ABO12303).Surfactant protein D was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Surfactant protein D Antibody (ABO12303) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
  • IHC - Anti-SP-D Antibody ABO12303
    Figure 5. IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (ABO12303).Surfactant protein D was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Surfactant protein D Antibody (ABO12303) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. 5
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, ICC, E
Primary Accession P35247
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Pulmonary surfactant-associated protein D(SFTPD) detection. Tested with WB, IHC-P, IHC-F, ICC, ELISA in Human;Mouse; Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 6441
Other Names Pulmonary surfactant-associated protein D, PSP-D, SP-D, Collectin-7, Lung surfactant protein D, SFTPD, COLEC7, PSPD, SFTP4
Calculated MW 37728 MW KDa
Application Details ELISA , 0.1-0.5 µg/ml
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, By Heat
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml
Immunocytochemistry, 0.5-1 µg/ml
Western blot, 0.1-0.5 µg/ml
Subcellular Localization Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
Tissue Specificity Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes). .
Protein Name Pulmonary surfactant-associated protein D
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Surfactant protein D (292-321aa RSAAENAALQQLVVAKNEAAFLSMTD SKTE), different from the related mouse and rat sequences by eight amino acids.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the SFTPD family.
Protein Information
Name SFTPD
Synonyms COLEC7, PSPD, SFTP4
Function Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
Cellular Location Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film
Tissue Location Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes).
Research Areas
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Background

Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. It is mapped to 10q22.2-q23.1. The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism.

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$ 280.00
Cat# ABO12303
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