|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for Interferon-inducible protein AIM2(AIM2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Interferon-inducible protein AIM2, Absent in melanoma 2, AIM2|
|Calculated MW||38954 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat|
Western blot, 0.1-0.5 µg/ml, Human
|Subcellular Localization||Nucleus. Cytoplasm. Activated inflammasomes can aggregate in the cytosol as speck-like particles.|
|Tissue Specificity||Expressed in spleen, small intestine, peripheral blood leukocytes, and testis. .|
|Protein Name||Interferon-inducible protein AIM2|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E.coli-derived human AIM2 recombinant protein (Position: L14-H215). Human AIM2 shares 53.8% amino acid (aa) sequence identity with mouse AIM2.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Function||Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8 (By similarity). Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.|
|Cellular Location||Nucleus. Cytoplasm. Note=Activated inflammasomes can aggregate in the cytosol as speck-like particles|
|Tissue Location||Expressed in spleen, small intestine, peripheral blood leukocytes, and testis|
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Interferon-inducible protein AIM2,Â also known asÂ absent in melanoma 2Â or simplyÂ AIM2,Â is aÂ proteinÂ that in humans is encoded by theÂ AIM2Â gene. It is mapped to 1q22. AIM2 is a member of theÂ Ifi202/IFI16Â family. It plays a putative role in tumorigenic reversion and may control cell proliferation.Â Interferon-gammaÂ induces expression of AIM2. Though there has been virtually no biochemistry performed, a model based on cell-based or in vivo experiments has led to the current model of how AIM2 triggers the inflammasome. The C-terminal HIN domain binds double stranded DNAÂ (either viral, bacterial, or even host) and acts as a cytosolic dsDNA sensor. This leads to the oligomerization of the inflammasome complex. The N-terminal pyrin domain of AIM2 interacts with the pyrin domain of another proteinÂ ASCÂ (or Apoptosis-associated Speck-like protein containing a caspase activation and recruitment domain). ASC also contains aÂ CARD domainÂ (caspase activation and recruitment domain), that recruits procaspase-1 to the complex. This leads to the autoactivation ofÂ caspase-1, an enzyme that processes proinflammatoryÂ cytokinesÂ (IL-1bÂ andÂ IL-18).
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