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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-CD22 Picoband Antibody   

Anti-CD22 Picoband Antibody

     
  • WB - Anti-CD22 Picoband Antibody ABO12377
    Anti- CD22 Picoband antibody, ABO12377, Western blottingAll lanes: Anti CD22 (ABO12377) at 0.5ug/mlLane 1: MCF-7 Whole Cell Lysate at 40ugLane 2: 22RV1 Whole Cell Lysate at 40ugPredicted bind size: 95KDObserved bind size: 95KD
  • IHC - Anti-CD22 Picoband Antibody ABO12377
    Anti- CD22 Picoband antibody, ABO12377, IHC(P)IHC(P): Human Tonsil Tissue
  • FC - Anti-CD22 Picoband Antibody ABO12377
    Figure 3. Flow Cytometry analysis of Raji cells using anti-CD22 antibody (ABO12377).Overlay histogram showing Raji cells stained with ABO12377 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD22 Antibody (ABO12377,1μg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, FC, ICC
Primary Accession P20273
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for B-cell receptor CD22(CD22) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 933
Other Names B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, Sialic acid-binding Ig-like lectin 2, Siglec-2, T-cell surface antigen Leu-14, CD22, CD22, SIGLEC2
Calculated MW 95348 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, By Heat
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml

Immunocytochemistry, 0.5-1 µg/ml
Western blot, 0.1-0.5 µg/ml
Flow Cytometry, 1-3μg/1x106cells
Subcellular Localization Cell membrane; Single-pass type I membrane protein.
Tissue Specificity B-lymphocytes.
Protein Name B-cell receptor CD22
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human CD22 (696-724aa LAILILAICGLKLQRRWKRTQSQQGLQEN), different from the related mouse sequence by ten amino acids.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name CD22
Synonyms SIGLEC2
Function Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location B-lymphocytes.
Research Areas
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Background

CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor cross-linking. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. The B cell coreceptor CD22 plays an important role in regulating signal transduction via the B cell Ag receptor. And CD22 is located within the band region q13.1 of chromosome 19.

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$ 280.00
Cat# ABO12377
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