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Anti-FXYD1 Picoband Antibody

     
  • WB - Anti-FXYD1 Picoband Antibody ABO12390
    Anti- FXYZ1 Picoband antibody, ABO12390, Western blottingAll lanes: Anti FXYZ1 (ABO12390) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Cardiac Muscle Tissue Lysate at 50ugLane 3: Mouse Cardiac Muscle Tissue Lysate at 50ugLane 4: HEPG2 Whole Cell Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugPredicted bind size: 10KDObserved bind size: 10KD
  • IHC - Anti-FXYD1 Picoband Antibody ABO12390
    Anti- FXYZ1 Picoband antibody, ABO12390, IHC(P)IHC(P): Mouse Cardiac Muscle Tissue
  • IHC - Anti-FXYD1 Picoband Antibody ABO12390
    Anti- FXYZ1 Picoband antibody, ABO12390, IHC(P)IHC(P): Rat Cardiac Muscle Tissue
  • IHC - Anti-FXYD1 Picoband Antibody ABO12390
    Anti- FXYZ1 Picoband antibody, ABO12390, IHC(P)IHC(P): Human Prostatic Cancer Tissue
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession O00168
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Phospholemman(FXYD1) detection. Tested with WB, IHC-P in Human;Rat;Mouse.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 5348
Other Names Phospholemman, FXYD domain-containing ion transport regulator 1, FXYD1, PLM
Calculated MW 10441 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Membrane; Single-pass type I membrane protein.
Tissue Specificity Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis.
Protein Name Phospholemman
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human FXYD1 recombinant protein (Position: E21-R92). Human FXYD1 shares 86.1% and 87.5% amino acid (aa) sequence identity with mouse and rat FXYD1, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name FXYD1 (HGNC:4025)
Function Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which transports Na(+) out of the cell and K(+) into the cell. Inhibits NKA activity in its unphosphorylated state and stimulates activity when phosphorylated. Reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated inhibition of ATP1B1. Contributes to female sexual development by maintaining the excitability of neurons which secrete gonadotropin-releasing hormone.
Cellular Location Cell membrane, sarcolemma {ECO:0000250|UniProtKB:P56513}; Single-pass type I membrane protein. Apical cell membrane {ECO:0000250|UniProtKB:O08589}; Single-pass type I membrane protein. Membrane, caveola {ECO:0000250|UniProtKB:O08589}. Cell membrane, sarcolemma, T- tubule {ECO:0000250|UniProtKB:O08589}. Note=Detected in the apical cell membrane in brain. In myocytes, localizes to sarcolemma, t- tubules and intercalated disks. {ECO:0000250|UniProtKB:O08589}
Tissue Location Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis.
Citations (0)

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Background

Phospholemman is a protein that in humans is encoded by the FXYD1 gene. This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. Transcript variants with different 5' UTR sequences have been described in the literature.

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$ 280.00
Cat# ABO12390
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