|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for Microsomal glutathione S-transferase 1(MGST1) detection. Tested with WB in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Microsomal glutathione S-transferase 1, Microsomal GST-1, 188.8.131.52, Microsomal GST-I, MGST1, GST12, MGST|
|Calculated MW||17599 MW KDa|
|Application Details||Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat|
|Subcellular Localization||Microsome . Mitochondrion outer membrane ; Peripheral membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein .|
|Tissue Specificity||Highly expressed in liver.|
|Protein Name||Microsomal glutathione S-transferase 1|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human MGST1 (42-75aa KVFANPEDCVAFGKGENAKKYLRTDDRVERVRRA), different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins.|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Function||Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.|
|Cellular Location||Microsome. Mitochondrion outer membrane; Peripheral membrane protein Endoplasmic reticulum membrane; Multi-pass membrane protein|
|Tissue Location||Highly expressed in liver.|
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Provided below are standard protocols that you may find useful for product applications.
Microsomal glutathione S-transferase 1 is an enzyme that in humans is encoded by the MGST1 gene. The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Several transcript variants, some non-protein coding and some protein coding, have been found for this gene.
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