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Anti-Actin Picoband Antibody

     
  • WB - Anti-Actin Picoband Antibody ABO12437
    Anti- Actin Picoband antibody, ABO12437, Western blottingAll lanes: Anti Actin (ABO12437) at 0.5ug/mlLane 1: Rat Spleen Tissue Lysate at 50ugLane 2: Mouse Spleen Tissue Lysate at 50ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 42KDObserved bind size: 42KD
  • IHC - Anti-Actin Picoband Antibody ABO12437
    Anti- Actin Picoband antibody, ABO12437,IHC(P)IHC(P): Mouse Spleen Tissue
  • IHC - Anti-Actin Picoband Antibody ABO12437
    Anti- Actin Picoband antibody, ABO12437,IHC(P)IHC(P): Rat Spleen Tissue
  • IHC - Anti-Actin Picoband Antibody ABO12437
    Anti- Actin Picoband antibody, ABO12437,IHC(P)IHC(P): Human Mammary Cancer Tissue
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P68133
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Actin, alpha skeletal muscle(ACTA1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 58
Other Names Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA
Calculated MW 42051 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Cytoplasm, cytoskeleton.
Protein Name Actin, alpha skeletal muscle
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (277-308aa HETTYNSIMKCDIDIRKDLYANNVMSGGTTMY), identical to the related mouse and rat sequences.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins.
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name ACTA1
Synonyms ACTA
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
Citations (0)

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Background

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

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$ 280.00
Cat# ABO12437
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