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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Anti-Bik Picoband Antibody   

Anti-Bik Picoband Antibody

     
  • WB - Anti-Bik Picoband Antibody ABO12441
    Anti- Bik Picoband antibody, ABO12441, Western blottingAll lanes: Anti Bik (ABO12441) at 0.5ug/mlLane 1: Rat Spleen Tissue Lysate at 50ugLane 2: Rat Gaster Tissue Lysate at 50ugLane 3: Rat Intestine Tissue Lysate at 50ugLane 4: MCF-7 Whole Cell Lysate at 40ugLane 5: A549 Whole Cell Lysate at 40ugLane 6: SKOV Whole Cell Lysate at 40ugPredicted bind size: 22KDObserved bind size: 22KD
  • IHC - Anti-Bik Picoband Antibody ABO12441
    Anti- Bik Picoband antibody, ABO12441, IHC(P)IHC(P): Mouse Spleen Tissue
  • IHC - Anti-Bik Picoband Antibody ABO12441
    Anti- Bik Picoband antibody, ABO12441, IHC(P)IHC(P): Rat Spleen Tissue
  • IHC - Anti-Bik Picoband Antibody ABO12441
    Anti- Bik Picoband antibody, ABO12441, IHC(P)IHC(P): Human Thyroid Cancer Tissue
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q13323
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Bcl-2-interacting killer(BIK) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 638
Other Names Bcl-2-interacting killer, Apoptosis inducer NBK, BIP1, BP4, BIK, NBK
Calculated MW 18016 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Human, Rat
Subcellular Localization Endomembrane system; Single-pass membrane protein. Mitochondrion membrane ; Single-pass membrane protein . Around the nuclear envelope, and in cytoplasmic membranes.
Protein Name Bcl-2-interacting killer
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human Bik recombinant protein (Position: M1-R123). Human Bik shares 42.3% amino acid (aa) sequence identity with mouse Bik.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins.
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name BIK
Synonyms NBK
Function Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
Cellular Location Endomembrane system; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Note=Around the nuclear envelope, and in cytoplasmic membranes
Research Areas
Citations (0)

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Background

Bcl-2-interacting killer is a protein that in humans is encoded by the BIK gene. This gene is mapped to 22q13.31. The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins.

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$ 280.00
Cat# ABO12441
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