|Description||Rabbit IgG polyclonal antibody for Protection of telomeres protein 1(POT1) detection. Tested with WB in Human.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Protection of telomeres protein 1, hPot1, POT1-like telomere end-binding protein, POT1|
|Calculated MW||71442 MW KDa|
|Application Details||Western blot, 0.1-0.5 µg/ml, Human|
|Subcellular Localization||Nucleus . Chromosome, telomere . Colocalizes with telomeric DNA.|
|Tissue Specificity||Ubiquitous. .|
|Protein Name||Protection of telomeres protein 1|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human POT1 (195-234aa RVLIQDLVLEGDLSHIHRLQNLTIDILVYDNHVHVARSLK), different from the related mouse sequence by eleven amino acids.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins.|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Function||Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA- binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'- TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity.|
|Cellular Location||Nucleus. Chromosome, telomere Note=Colocalizes with telomeric DNA|
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Provided below are standard protocols that you may find useful for product applications.
Protection of telomeres protein 1 is a protein that in humans is encoded by the POT1 gene. This gene is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described.
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