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Anti-RAB10 Picoband Antibody

     
  • WB - Anti-RAB10 Picoband Antibody ABO12474
    Anti- RAB10 Picoband antibody, ABO12474, Western blottingAll lanes: Anti RAB10 (ABO12474) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Thymus Tissue Lysate at 50ugLane 3: Mouse Brain Tissue Lysate at 50ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: MCF-7 Whole Cell Lysate at 40ugLane 6: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 22KDObserved bind size: 22KD
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P61026
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Ras-related protein Rab-10(RAB10) detection. Tested with WB in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 10890
Other Names Ras-related protein Rab-10, RAB10
Calculated MW 22541 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Cytoplasmic vesicle membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane . Golgi apparatus, trans-Golgi network membrane . Endosome membrane . Recycling endosome membrane . Cytoplasmic vesicle, phagosome membrane . Cell projection, cilium . Endoplasmic reticulum membrane . Associates with SLC2A4/GLUT4 storage vesicles (PubMed:22908308). Localizes to the base of the cilium (PubMed:20576682). Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (PubMed:23263280). .
Protein Name Ras-related protein Rab-10
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human RAB10 (153-185aa AKANINIEKAFLTLAEDILRKTPVKEPNSENVD), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins.
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name RAB10
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. According to PubMed:23263280, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.
Cellular Location Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane. Golgi apparatus, trans-Golgi network membrane {ECO:0000250|UniProtKB:P24409} Endosome membrane. Recycling endosome membrane {ECO:0000250|UniProtKB:P24409}. Cytoplasmic vesicle, phagosome membrane {ECO:0000250|UniProtKB:P24409}. Cell projection, cilium. Endoplasmic reticulum membrane. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:P61027}. Note=Associates with SLC2A4/GLUT4 storage vesicles (PubMed:22908308). Localizes to the base of the cilium (PubMed:20576682). Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (PubMed:23263280) {ECO:0000250|UniProtKB:P24409, ECO:0000269|PubMed:20576682, ECO:0000269|PubMed:22908308, ECO:0000269|PubMed:23263280}
Research Areas
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Background

Ras-related protein Rab-10 is a protein that in humans is encoded by the RAB10 gene. RAB10 belongs to the RAS superfamily of small GTPases. And RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking. The RAB10 gene is mapped to chromosome 2p23.1-p22.3 by radiation hybrid analysis. It was found that the purified recombinant GAP domain of human AS160 showed GAP activity with RAB2A, RAB8A, RAB10, and RAB14, but not with 14 other RABs. Immunoblot analysis showed that these RABs associated with Glut4-positive vesicles in mouse adipocytes. Thereby, it concluded that AK160 functions as a RAB GAP and that RABs may participate in GLUT4 translocation.

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$ 240.00
Cat# ABO12474
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