|Description||Rabbit IgG polyclonal antibody for Thymidine kinase, cytosolic(TK1) detection. Tested with WB in Human.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Thymidine kinase, cytosolic, 18.104.22.168, TK1|
|Calculated MW||25469 MW KDa|
|Application Details||Western blot, 0.1-0.5 µg/ml, Human|
|Protein Name||Thymidine kinase, cytosolic|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E. coli-derived human TK1 recombinant protein (Position: S2-N234). Human TK1 shares 87.1% and 91.7% amino acid (aa) sequence identity with mouse and rat TK1, respectively.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
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Provided below are standard protocols that you may find useful for product applications.
Thymidine kinase 1, soluble (gene name TK1), is a human thymidine kinase. Thymidine kinase catalyzes the phosphorylation of thymidine to deoxythymidine monophosphate. This gene is mapped to 17q23.2-q25.3. It is estimated that the maximal size of the TK1 gene is 14 kb and its minimal size between 4 and 5 kb. The gene contains many noncoding inserts and numerous Alu sequences. There are two forms of this protein, which have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
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