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Anti-CFD Antibody

     
  • WB - Anti-CFD Antibody ABO12536
    Western blot analysis of Factor D expression in mouse spleen extract (lane 1). Factor D at 43KD was detected using rabbit anti- Factor D Antigen Affinity purified polyclonal antibody (Catalog # ABO12536) at0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P03953
Host Rabbit
Reactivity Mouse
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Complement factor D(CFD) detection. Tested with WB in Mouse.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 11537
Other Names Complement factor D, 3.4.21.46, 28 kDa adipocyte protein, Adipsin, C3 convertase activator, Properdin factor D, Cfd, Adn, Df
Calculated MW 28057 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Mouse
Subcellular Localization Secreted.
Protein Name Complement factor D
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived mouse Factor D recombinant protein (Position: Q30-K235). Mouse Factor D shares 66.5% and 87.4% amino acid (aa) sequence identity with human and rat Factor D, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins.
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name Cfd
Synonyms Adn, Df
Function Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
Cellular Location Secreted.
Research Areas
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Background

Factor D is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. It is also a serine protease that is secreted by adipocytes into the bloodstream. And it stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology.

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$ 240.00
Cat# ABO12536
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