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>   home   >   Products   >   Primary Antibodies   >   Metabolism   >   Anti-Diablo/SMAC Picoband™ Antibody   

Anti-Diablo/SMAC Picoband™ Antibody

     
  • WB - Anti-Diablo/SMAC Picoband™  Antibody ABO12546
    Western blot analysis of Smac/Diablo expression in rat testis extract (lane 1), 22RV1 whole cell lysates (lane 2) and SKOV whole cell lysates (lane 3). Smac/Diablo at 25KD was detected using rabbit anti- Smac/Diablo Antigen Affinity purified polyclonal antibody (Catalog # ABO12546) at0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method .
  • IHC - Anti-Diablo/SMAC Picoband™  Antibody ABO12546
    Smac/Diablo was detected in paraffin-embedded sections of mouse testis tissues using rabbit anti- Smac/Diablo Antigen Affinity purified polyclonal antibody (Catalog # ABO12546) at 1 ??g/mL. The immunohistochemical section was developed using SABC method .
  • IHC - Anti-Diablo/SMAC Picoband™  Antibody ABO12546
    Smac/Diablo was detected in paraffin-embedded sections of rat testis tissues using rabbit anti- Smac/Diablo Antigen Affinity purified polyclonal antibody (Catalog # ABO12546) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
  • IHC - Anti-Diablo/SMAC Picoband™  Antibody ABO12546
    Smac/Diablo was detected in paraffin-embedded sections of human testis tissues using rabbit anti- Smac/Diablo Antigen Affinity purified polyclonal antibody (Catalog # ABO12546) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9NR28
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Diablo homolog, mitochondrial(DIABLO) detection. Tested with WB, IHC-P, ELISA in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 56616
Other Names Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase, Smac, DIABLO, SMAC
Calculated MW 27131 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

ELISA , 0.1-0.5 µg/ml, Human, -
Western blot, 0.1-0.5 µg/ml, Human, Rat
Subcellular Localization Mitochondrion . Released into the cytosol when cells undergo apoptosis.
Tissue Specificity Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. .
Protein Name Diablo homolog, mitochondrial
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239). Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with mouse Smac/Diablo.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins.
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name DIABLO
Synonyms SMAC
Function Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
Cellular Location Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis
Tissue Location Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed
Research Areas
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Background

Diablo homolog (DIABLO), also referred to as second mitochondria-derived activator of caspases or SMAC, is a mitochondrial protein that in humans is encoded by the DIABLO (direct IAP binding protein with low pI) gene on chromosome 12. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis.  A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.

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$ 280.00
Cat# ABO12546
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