|Application ||WB, IHC-P, ICC|
|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for Fatty acid synthase(FASN) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Fatty acid synthase, 184.108.40.206, [Acyl-carrier-protein] S-acetyltransferase, 220.127.116.11, [Acyl-carrier-protein] S-malonyltransferase, 18.104.22.168, 3-oxoacyl-[acyl-carrier-protein] synthase, 22.214.171.124, 3-oxoacyl-[acyl-carrier-protein] reductase, 126.96.36.199, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, 188.8.131.52, Enoyl-[acyl-carrier-protein] reductase, 184.108.40.206, Oleoyl-[acyl-carrier-protein] hydrolase, 220.127.116.11, FASN, FAS|
|Calculated MW||273427 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, By Heat|
Western blot, 0.1-0.5 µg/ml
|Subcellular Localization||Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.|
|Tissue Specificity||Ubiquitous. Prominent expression in brain, lung, and liver. .|
|Protein Name||Fatty acid synthase|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins.|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Function||Fatty acid synthetase catalyzes the formation of long- chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities as an acyl carrier protein.|
|Cellular Location||Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV|
|Tissue Location||Ubiquitous. Prominent expression in brain, lung, and liver.|
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Provided below are standard protocols that you may find useful for product applications.
Fatty acid synthaseÂ (FAS) is anÂ enzymeÂ that in humans is encoded by theÂ FASNÂ gene. It is mapped to 17q25. The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
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