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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-FASN Picoband Antibody   

Anti-FASN Picoband Antibody

     
  • WB - Anti-FASN Picoband Antibody ABO12551
    Western blot analysis of FASN expression in HEPG2 whole cell lysates (lane 1), U87 whole cell lysates (lane 2) and RAJI whole cell lysates (lane 3). FASN at 273KD was detected using rabbit anti- FASN Antigen Affinity purified polyclonal antibody (Catalog # ABO12551) at0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
  • IHC - Anti-FASN Picoband Antibody ABO12551
    FASN was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- FASN Antigen Affinity purified polyclonal antibody (Catalog # ABO12551) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA2010).
  • IHC - Anti-FASN Picoband Antibody ABO12551
    Figure 3. IHC analysis of FASN using anti-FASN antibody (ABO12551).FASN was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FASN Antibody (ABO12551) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
  • IHC - Anti-FASN Picoband Antibody ABO12551
    Figure 4. IHC analysis of FASN using anti-FASN antibody (ABO12551).FASN was detected in immunocytochemical section of MCF-7 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FASN Antibody (ABO12551) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, ICC
Primary Accession P49327
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Fatty acid synthase(FASN) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 2194
Other Names Fatty acid synthase, 2.3.1.85, [Acyl-carrier-protein] S-acetyltransferase, 2.3.1.38, [Acyl-carrier-protein] S-malonyltransferase, 2.3.1.39, 3-oxoacyl-[acyl-carrier-protein] synthase, 2.3.1.41, 3-oxoacyl-[acyl-carrier-protein] reductase, 1.1.1.100, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, 4.2.1.59, Enoyl-[acyl-carrier-protein] reductase, 1.3.1.39, Oleoyl-[acyl-carrier-protein] hydrolase, 3.1.2.14, FASN, FAS
Calculated MW 273427 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, By Heat
Immunocytochemistry,0.5-1 µg/ml

Western blot, 0.1-0.5 µg/ml
Subcellular Localization Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tissue Specificity Ubiquitous. Prominent expression in brain, lung, and liver. .
Protein Name Fatty acid synthase
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins.
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name FASN
Synonyms FAS
Function Fatty acid synthetase catalyzes the formation of long- chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities as an acyl carrier protein.
Cellular Location Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Tissue Location Ubiquitous. Prominent expression in brain, lung, and liver.
Research Areas
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Background

Fatty acid synthase (FAS) is an enzyme that in humans is encoded by the FASN gene. It is mapped to 17q25. The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

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$ 280.00
Cat# ABO12551
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