|Description||Rabbit IgG polyclonal antibody for MAX gene-associated protein(MGA) detection. Tested with WB in Human.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||MAX gene-associated protein, MAX dimerization protein 5, MGA, KIAA0518, MAD5|
|Calculated MW||331836 MW KDa|
|Application Details||Western blot, 0.1-0.5 µg/ml, Human|
|Subcellular Localization||Nucleus .|
|Protein Name||MAX gene-associated protein|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MGA (2376-2415aa QKEAEAFAYYRRTHTANERRRRGEMRDLFEKLKITLGLLH), different from the related mouse sequence by two amino acids.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins.|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Function||Functions as a dual-specificity transcription factor, regulating the expression of both MAX-network and T-box family target genes. Functions as a repressor or an activator. Binds to 5'-AATTTCACACCTAGGTGTGAAATT-3' core sequence and seems to regulate MYC-MAX target genes. Suppresses transcriptional activation by MYC and inhibits MYC-dependent cell transformation. Function activated by heterodimerization with MAX. This heterodimerization serves the dual function of both generating an E-box-binding heterodimer and simultaneously blocking interaction of a corepressor (By similarity).|
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MgaÂ is a DNA-bindingÂ proteinÂ that activates theÂ expressionÂ of several importantÂ virulenceÂ genesÂ inÂ Streptococcus pyogenesÂ (group AÂ Streptococcus, GAS) in response to changing environmental conditions. It had been found that the mouse transcription factor Max interacted withÂ Mga.Â Coimmunoprecipitation analysis confirmed that Max andÂ MgaÂ interacted in transfected HEK293 cells. EMSA revealed thatÂ MgaÂ required Max for binding to the E-box sequence CACGTG. The isolated T-box ofÂ MgaÂ bound the brachyury T-box-binding site in the absence of Max.Â MgaÂ repressed transcription of a reporter driven from a T-box-binding site, but coexpression ofÂ MgaÂ with Max caused transcriptional activation from the T-box-binding site. Expression ofÂ MgaÂ with Max, but notÂ MgaÂ alone, activated transcription from a reporter containing the E-box site.
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