|Application ||WB, IHC-P, E|
|Description||Rabbit IgG polyclonal antibody for Nucleobindin-2(NUCB2) detection. Tested with WB, IHC-P, ELISA in Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Nucleobindin-2, DNA-binding protein NEFA, Prepronesfatin, Nesfatin-1, Nucb2, Nefa|
|Calculated MW||50304 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Mouse, Rat, By Heat|
ELISA , 0.1-0.5 µg/ml, Mouse, -
Western blot, 0.1-0.5 µg/ml, Mouse
|Subcellular Localization||Cytoplasm . Perikaryon . Endoplasmic reticulum . Golgi apparatus . Nucleus envelope . Membrane ; Peripheral membrane protein . Secreted . In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm in neurons. In cell culture, cytoplasmic and secreted.|
|Tissue Specificity||Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata. Nucb2 and necdin levels were higher in postmitotic neurons. .|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E. coli-derived mouse Nucleobindin 2 recombinant protein (Position: V25-L106). Mouse Nucleobindin 2 shares 85.4% and 97.6% amino acid (aa) sequence identity with human and rat Nucleobindin 2, respectively.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins.|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Function||Calcium-binding protein. May have a role in calcium homeostasis.|
|Cellular Location||Cytoplasm. Perikaryon. Endoplasmic reticulum. Golgi apparatus Nucleus envelope. Membrane; Peripheral membrane protein. Secreted. Note=In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm in neurons In cell culture, cytoplasmic and secreted|
|Tissue Location||Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata Nucb2 and necdin levels were higher in postmitotic neurons|
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Nucleobindin-2Â is aÂ calcium-bindingÂ EF-hand proteinÂ that in humans is encoded by theÂ NUCB2Â gene. By exon trapping and sequence analysis,Â theÂ NUCB2Â sequence has been identified on chromosome 11p15-p14. This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells.
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