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Anti-Nesfatin-1 Picoband Antibody

     
  • WB - Anti-Nesfatin-1 Picoband Antibody ABO12562
    Western blot analysis of Nucleobindin 2 expression in HEPA whole cell lysates (lane 1). Nucleobindin 2 at 55KD was detected using rabbit anti- Nucleobindin 2 Antigen Affinity purified polyclonal antibody (Catalog # ABO12562) at0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method .
  • IHC - Anti-Nesfatin-1 Picoband Antibody ABO12562
    Nucleobindin 2 was detected in paraffin-embedded sections of mouse gaster tissues using rabbit anti- Nucleobindin 2 Antigen Affinity purified polyclonal antibody (Catalog # ABO12562) at 1 ??g/mL. The immunohistochemical section was developed using SABC method .
  • IHC - Anti-Nesfatin-1 Picoband Antibody ABO12562
    Nucleobindin 2 was detected in paraffin-embedded sections of rat gaster tissues using rabbit anti- Nucleobindin 2 Antigen Affinity purified polyclonal antibody (Catalog # ABO12562) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P81117
Host Rabbit
Reactivity Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Nucleobindin-2(NUCB2) detection. Tested with WB, IHC-P, ELISA in Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 53322
Other Names Nucleobindin-2, DNA-binding protein NEFA, Prepronesfatin, Nesfatin-1, Nucb2, Nefa
Calculated MW 50304 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Mouse, Rat, By Heat

ELISA , 0.1-0.5 µg/ml, Mouse, -
Western blot, 0.1-0.5 µg/ml, Mouse
Subcellular Localization Cytoplasm . Perikaryon . Endoplasmic reticulum . Golgi apparatus . Nucleus envelope . Membrane ; Peripheral membrane protein . Secreted . In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm in neurons. In cell culture, cytoplasmic and secreted.
Tissue Specificity Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata. Nucb2 and necdin levels were higher in postmitotic neurons. .
Protein Name Nucleobindin-2
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived mouse Nucleobindin 2 recombinant protein (Position: V25-L106). Mouse Nucleobindin 2 shares 85.4% and 97.6% amino acid (aa) sequence identity with human and rat Nucleobindin 2, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins.
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name Nucb2
Synonyms Nefa
Function Calcium-binding protein. May have a role in calcium homeostasis.
Cellular Location Cytoplasm. Perikaryon. Endoplasmic reticulum. Golgi apparatus Nucleus envelope. Membrane; Peripheral membrane protein. Secreted. Note=In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm in neurons In cell culture, cytoplasmic and secreted
Tissue Location Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata Nucb2 and necdin levels were higher in postmitotic neurons
Research Areas
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Background

Nucleobindin-2 is a calcium-binding EF-hand protein that in humans is encoded by the NUCB2 gene. By exon trapping and sequence analysis, the NUCB2 sequence has been identified on chromosome 11p15-p14. This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells.

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$ 280.00
Cat# ABO12562
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