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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-CD9 Picoband Antibody   

Anti-CD9 Picoband Antibody

     
  • WB - Anti-CD9 Picoband Antibody ABO12615
    Western blot analysis of CD9 expression in mouse kidney extract (lane 1). CD9 at 25KD was detected using rabbit anti- CD9 Antigen Affinity purified polyclonal antibody (Catalog # ABO12615) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
  • IHC - Anti-CD9 Picoband Antibody ABO12615
    CD9 was detected in paraffin-embedded sections of mouse spleen tissues using rabbit anti- CD9 Antigen Affinity purified polyclonal antibody (Catalog # ABO12615) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P40240
Host Rabbit
Reactivity Mouse
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for CD9 antigen(CD9) detection. Tested with WB, IHC-P in Mouse.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 12527
Other Names CD9 antigen, CD9, Cd9
Calculated MW 25258 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Mouse, By Heat

Western blot, 0.1-0.5 µg/ml, Mouse
Subcellular Localization Membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Secreted, exosome .
Tissue Specificity Expressed predominantly in the peripheral nervous system. .
Protein Name CD9 antigen
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived mouse CD9 recombinant protein (Position: T110-I193). Mouse CD9 shares 77.4% and 86.9% amino acid (aa) sequence identity with human and rat CD9, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name Cd9 {ECO:0000303|PubMed:8236164, ECO:0000312|MGI:MGI:88348}
Function Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:10700183, PubMed:10634790, PubMed:10634791, PubMed:14715942). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (PubMed:10700183, PubMed:10634790, PubMed:10634791, PubMed:21690351). Acts as a receptor for PSG17 (PubMed:11805154). Involved in platelet activation and aggregation (PubMed:14715942). Regulates paranodal junction formation (PubMed:14715942). Involved in cell adhesion, cell motility and tumor metastasis (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Secreted, exosome. Note=Present at the cell surface of oocytes (PubMed:10518536, PubMed:10634791, PubMed:23213457) Accumulates in the adhesion area between the sperm and egg following interaction between IZUMO1 and its receptor IZUMO1R/JUNO (PubMed:25209248).
Tissue Location Expressed predominantly in the peripheral nervous system (PubMed:14715942). Highly expressed in oocytes and blastocysts (at protein level) (PubMed:10518536, PubMed:10634790, PubMed:10634791, PubMed:23213457). Expression is also observed on follicular oocytes in the ovary, whereas no expression is found on follicular cells (at protein level) (PubMed:10518536, PubMed:10634790).
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Background

CD9 antigen is a protein that in humans is encoded by the CD9 gene. CD9 is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is found on the surface of exosomes. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance. This protein also seems to be a key part in the egg-sperm fusion during mammalian fertilization. While oocytes are ovulated, CD9-deficient oocytes are not properly fused with sperm upon fertilization. CD9 is located in the microvillar membrane of the oocytes and also appears to intervene in maintaining the normal shape of oocyte microvilli.

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$ 280.00
Cat# ABO12615
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