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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-TNFRSF7/CD27 Picoband Antibody   

Anti-TNFRSF7/CD27 Picoband Antibody

     
  • WB - Anti-TNFRSF7/CD27 Picoband Antibody ABO12616
    Western blot analysis of CD27 expression in HELA whole cell lysates (lane 1). CD27 at 51KD was detected using rabbit anti- CD27 Antigen Affinity purified polyclonal antibody (Catalog # ABO12616) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
  • IHC - Anti-TNFRSF7/CD27 Picoband Antibody ABO12616
    CD27 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- CD27 Antigen Affinity purified polyclonal antibody (Catalog # ABO12616) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
  • FC - Anti-TNFRSF7/CD27 Picoband Antibody ABO12616
    Figure 3. Flow Cytometry analysis of A549 cells using anti-CD27 antibody (ABO12616).Overlay histogram showing A549 cells stained with ABO12616 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD27 Antibody (ABO12616,1μg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, FC, ICC
Primary Accession P26842
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for CD27 antigen(CD27) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 939
Other Names CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, CD27, TNFRSF7
Calculated MW 29137 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, By Heat
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml

Immunocytochemistry, 0.5-1 µg/ml

Western blot, 0.1-0.5 µg/ml
Flow Cytometry, 1-3μg/1x106cells
Subcellular Localization Membrane; Single-pass type I membrane protein.
Tissue Specificity Found in most T-lymphocytes.
Protein Name CD27 antigen
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived human CD27 recombinant protein (Position: A20-R191). Human CD27 shares 62.8% amino acid (aa) sequence identity with mouse CD27.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name CD27
Synonyms TNFRSF7
Function Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Found in most T-lymphocytes.
Research Areas
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Background

CD27 is a member of the tumor necrosis factor receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. And this receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

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$ 280.00
Cat# ABO12616
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