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Anti-CYP11A1 Picoband Antibody

     
  • WB - Anti-CYP11A1 Picoband Antibody ABO12627
    Western blot analysis of CYP11A1 expression in rat brain extract (lane 1), rat kidney extract (lane 2) and 293T whole cell lysates (lane 3). CYP11A1 at 75KD was detected using rabbit anti-CYP11A1 Antigen Affinity purified polyclonal antibody (Catalog # ABO12627) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P05108
Host Rabbit
Reactivity Human, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Cholesterol side-chain cleavage enzyme, mitochondrial(CYP11A1) detection. Tested with WB in Human;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 1583
Other Names Cholesterol side-chain cleavage enzyme, mitochondrial, 1.14.15.6, CYPXIA1, Cholesterol desmolase, Cytochrome P450 11A1, Cytochrome P450(scc), CYP11A1, CYP11A
Calculated MW 60102 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human, Rat
Subcellular Localization Mitochondrion membrane.
Protein Name Cholesterol side-chain cleavage enzyme, mitochondrial
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived human CYP11A1 recombinant protein (Position: M333-A418). Human CYP11A1 shares 89.5% amino acid (aa) sequence identity with both mouse and rat CYP11A1.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name CYP11A1
Synonyms CYP11A
Function Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone, the precursor of most steroid hormones.
Cellular Location Mitochondrion inner membrane {ECO:0000250|UniProtKB:P14137}; Peripheral membrane protein. Note=Localizes to the matrix side of the mitochondrion inner membrane. {ECO:0000250|UniProtKB:P14137}
Research Areas
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Background

CYP11A1 (Cytochrome p450, family 11, subfamily A, polypeptide 1), commonly referred to as P450scc, is a mitochondrial enzyme associated with the conversion of cholesterol to pregnenolone. CYP11A1 is a member of the cytochrome P450 superfamily of enzymes. The CYP11A1 gene is mapped on 15q24.1. Expression of the CYP11A1 gene may play a role in skin physiology and pathology and that cutaneous proopiomelanocortin activity may be autoregulated by a feedback mechanism involving glucocorticoids synthesized locally. Using in vitro studies, CYP11A1 catalyzed the side-chain cleavage of 7-dehydrocholesterol to form 7-dehydropregnenolone. In addition, CYP11A1 catalyzed the metabolism of biologically inert vitamin D3, which is formed from 7-dehydrocholesterol, to form 2 hydroxylated products, 20-hydroxyvitamin D3 and 20, 22-dihydroxyvitamin D3. Mutations in the CYP11A1 gene cause congenital adrenal insufficiency with partial or complete 46, XY sex reversal.

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$ 240.00
Cat# ABO12627
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