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Anti-EAAT1 Picoband Antibody

     
  • WB - Anti-EAAT1 Picoband Antibody ABO12644
    Western blot analysis of EAAT1 expression in rat brain extract (lane 1) and mouse brain extract (lane 2). EAAT1 at 60KD was detected using rabbit anti-EAAT1 Antigen Affinity purified polyclonal antibody (Catalog # ABO12644) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method .
  • IHC - Anti-EAAT1 Picoband Antibody ABO12644
    EAAT1 was detected in paraffin-embedded sections of mouse cerebellum tissues using rabbit anti- EAAT1 Antigen Affinity purified polyclonal antibody (Catalog # ABO12644) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
  • IHC - Anti-EAAT1 Picoband Antibody ABO12644
    EAAT1 was detected in paraffin-embedded sections of rat brain tissues using rabbit anti- EAAT1 Antigen Affinity purified polyclonal antibody (Catalog # ABO12644) at 1 ??g/mL. The immunohistochemical section was developed using SABC method .
  • IHC - Anti-EAAT1 Picoband Antibody ABO12644
    EAAT1 was detected in paraffin-embedded sections of huaman glioma tissues using rabbit anti- EAAT1 Antigen Affinity purified polyclonal antibody (Catalog # ABO12644) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P43003
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Excitatory amino acid transporter 1(SLC1A3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 6507
Other Names Excitatory amino acid transporter 1, Sodium-dependent glutamate/aspartate transporter 1, GLAST-1, Solute carrier family 1 member 3, SLC1A3, EAAT1, GLAST, GLAST1
Calculated MW 59572 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Mouse, Rat, Human
Subcellular Localization Membrane; Multi-pass membrane protein.
Tissue Specificity Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
Protein Name Excitatory amino acid transporter 1
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human EAAT1 (14-42aa RMERFQQGVRKRTLLAKKKVQNITKEDVK), different from the related mouse sequence by three amino acids, and identical to the related rat sequence.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name SLC1A3
Function Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7521911, PubMed:8123008, PubMed:20477940, PubMed:26690923, PubMed:28032905, PubMed:28424515). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed:20477940). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed:20477940). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity).
Cellular Location Cell membrane; Multi- pass membrane protein
Tissue Location Detected in brain (PubMed:8218410, PubMed:7521911, PubMed:8123008). Detected at very much lower levels in heart, lung, placenta and skeletal muscle (PubMed:7521911, PubMed:8123008). Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia (PubMed:7521911).
Research Areas
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Background

Solute carrier family 1 (glial high-affinity glutamate transporter), member 3, also known as SLC1A3, EAAT1 or GLAST, is a protein that in humans is encoded by the SLC1A3 gene. This gene is a member of high affinity glutamate transporter family. It is mapped to chromosome 5p13.2 by fluorescence in situ hybridization (FISH). And this gene transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Furthermore, this gene acts as a symport by cotransporting sodium.

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$ 280.00
Cat# ABO12644
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