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Anti-HE4 Picoband Antibody

     
  • WB - Anti-HE4 Picoband Antibody ABO12648
    Western blot analysis of HE4 expression in 22RV1 whole cell lysates (lane 1). HE4 at 25KD was detected using rabbit anti- HE4 Antigen Affinity purified polyclonal antibody (Catalog # ABO12648) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q14508
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for WAP four-disulfide core domain protein 2(WFDC2) detection. Tested with WB, ELISA in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Other Names WAP four-disulfide core domain protein 2, Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2, HE4, WAP5
Calculated MW 12993 MW KDa
Application Details ELISA , 0.1-0.5 µg/ml, Human, -
Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Secreted .
Tissue Specificity Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. .
Protein Name WAP four-disulfide core domain protein 2
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived human HE4 recombinant protein (Position: E31-F124). Human HE4 shares 40.4% and 68% amino acid (aa) sequence identity with mouse and rat HE4, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name WFDC2
Synonyms HE4, WAP5
Function Broad range protease inhibitor.
Cellular Location Secreted
Tissue Location Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. EMBL; X63187; CAA44869.1; -; mRNA EMBL; AF330259; AAL37485.1; -; mRNA EMBL; AF330260; AAL37486.1; -; mRNA EMBL; AF330261; AAL37487.1; -; mRNA EMBL; AF330262; AAL37488.1; -; mRNA EMBL; AY212888; AAO52683.1; -; mRNA EMBL; CR456977; CAG33258.1; -; mRNA EMBL; AL031663; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; CH471077; EAW75836.1; -; Genomic_DNA EMBL; CH471077; EAW75837.1; -; Genomic_DNA EMBL; CH471077; EAW75839.1; -; Genomic_DNA EMBL; BC046106; AAH46106.1; -; mRNA CCDS; CCDS35501.1; -. [Q14508-1] PIR; S25454; S25454 RefSeq; NP_006094.3; NM_006103.3. [Q14508-1] UniGene; Hs.2719; - ProteinModelPortal; Q14508; - SMR; Q14508; - BioGrid; 115677; 14 IntAct; Q14508; 15 STRING; 9606.ENSP00000361761; - MEROPS; I17.004; - GlyConnect; 1902; - iPTMnet; Q14508; - BioMuta; WFDC2; - DMDM; 20141958; - EPD; Q14508; - MaxQB; Q14508; - PaxDb; Q14508; - PeptideAtlas; Q14508; - PRIDE; Q14508; - ProteomicsDB; 60010; - ProteomicsDB; 60011; -. [Q14508-2] ProteomicsDB; 60012; -. [Q14508-3] ProteomicsDB; 60013; -. [Q14508-4] ProteomicsDB; 60014; -. [Q14508-5] DNASU; 10406; - Ensembl; ENST00000217425; ENSP00000217425; ENSG00000101443. [Q14508-5] Ensembl; ENST00000339946; ENSP00000340215; ENSG00000101443. [Q14508-3] Ensembl; ENST00000342873; ENSP00000342890; ENSG00000101443. [Q14508-2] Ensembl; ENST00000372676; ENSP00000361761; ENSG00000101443. [Q14508-1] GeneID; 10406; - KEGG; hsa:10406; - UCSC; uc002xoo.4; human. [Q14508-1] CTD; 10406; - DisGeNET; 10406; - EuPathDB; HostDB:ENSG00000101443.17; - GeneCards; WFDC2; - HGNC; HGNC:15939; WFDC2 HPA; HPA042302; - MIM; 617548; gene neXtProt; NX_Q14508; - OpenTargets; ENSG00000101443; - PharmGKB; PA38059; - eggNOG; ENOG410J36N; Eukaryota eggNOG; ENOG41125F3; LUCA GeneTree; ENSGT00730000111410; - HOVERGEN; HBG018073; - InParanoid; Q14508; - OMA; DLNCTQE; - OrthoDB; 445432at2759; - PhylomeDB; Q14508; - ChiTaRS; WFDC2; human GeneWiki; WFDC2; - GenomeRNAi; 10406; - PRO; PR:Q14508; - Proteomes; UP000005640; Chromosome 20 Bgee; ENSG00000101443; Expressed in 113 organ(s), highest expression level in adult mammalian kidney ExpressionAtlas; Q14508; baseline and differential Genevisible; Q14508; HS GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB GO; GO:0005615; C:extracellular space; TAS:ProtInc GO; GO:0019828; F:aspartic-type endopeptidase inhibitor activity; IDA:CACAO GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW GO; GO:0004866; F:endopeptidase inhibitor activity; TAS:ProtInc GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:CACAO GO; GO:0006508; P:proteolysis; TAS:ProtInc GO; GO:0007283; P:spermatogenesis; TAS:ProtInc Gene3D; 4.10.75.10; -; 2 InterPro; IPR036645; Elafin-like_sf InterPro; IPR008197; WAP_dom Pfam; PF00095; WAP; 2 PRINTS; PR00003; 4DISULPHCORE SMART; SM00217; WAP; 2 SUPFAM; SSF57256; SSF57256; 2 PROSITE; PS51390; WAP; 2 1: Evidence at protein level; Alternative splicing; Aspartic protease inhibitor; Complete proteome; Disulfide bond; Glycoprotein; Protease inhibitor; Reference proteome; Repeat; Secreted; Serine protease inhibitor; Signal; Thiol protease inhibitor SIGNAL 1 30 CHAIN 31 124 WAP four-disulfide core domain protein 2 /FTId=PRO_0000041370 DOMAIN 31 73 WAP 1. {ECO:0000255|PROSITE- ProRule:PRU00722} DOMAIN 74 123 WAP 2. {ECO:0000255|PROSITE- ProRule:PRU00722} CARBOHYD 44 44 N-linked (GlcNAc...) asparagine DISULFID 36 62 {ECO:0000255|PROSITE-ProRule:PRU00722} DISULFID 45 66 {ECO:0000255|PROSITE-ProRule:PRU00722} DISULFID 49 61 {ECO:0000255|PROSITE-ProRule:PRU00722} DISULFID 55 70 {ECO:0000255|PROSITE-ProRule:PRU00722} DISULFID 80 110 {ECO:0000255|PROSITE-ProRule:PRU00722} DISULFID 93 114 {ECO:0000255|PROSITE-ProRule:PRU00722} DISULFID 97 109 {ECO:0000255|PROSITE-ProRule:PRU00722} DISULFID 103 119 {ECO:0000255|PROSITE-ProRule:PRU00722} VAR_SEQ 2 23 PACRLGPLAAALLLSLLLFGFT -> LQVQVNLPVSPLPTY PYSFFYP (in isoform 2) /FTId=VSP_007666 VAR_SEQ 24 74 Missing (in isoform 2) /FTId=VSP_007667 VAR_SEQ 27 74 Missing (in isoform 3) /FTId=VSP_007668 VAR_SEQ 71 79 SLPNDKEGS -> LLCPNGQLAE (in isoform 4) /FTId=VSP_007669 VAR_SEQ 75 102 DKEGSCPQVNINFPQLGLCRDQCQVDSQ -> ALFHWHLKT RRLWEISGPRPRRPTWDSS (in isoform 5) /FTId=VSP_007670 VAR_SEQ 80 124 Missing (in isoform 4) /FTId=VSP_007671 VAR_SEQ 103 124 Missing (in isoform 5) /FTId=VSP_007672 CONFLICT 71 72 SL -> LLC (in Ref. 1; CAA44869 and 2; AAL37485). CONFLICT 101 101 S -> T (in Ref. 1; CAA44869) SEQUENCE 124 AA; 12993 MW; 9536B00B385259AD CRC64; MPACRLGPLA AALLLSLLLF GFTLVSGTGA EKTGVCPELQ ADQNCTQECV SDSECADNLK CCSAGCATFC SLPNDKEGSC PQVNINFPQL GLCRDQCQVD SQCPGQMKCC RNGCGKVSCV TPNF
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Background

WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

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$ 240.00
Cat# ABO12648
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