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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-CRM1 Picoband Antibody   

Anti-CRM1 Picoband Antibody

     
  • WB - Anti-CRM1 Picoband Antibody ABO12651
    Western blot analysis of CRM1 expression in rat cardiac muscle extract (lane 1), mouse brain extract (lane 2) and A549 whole cell lysates (lane 3). CRM1 at 123KD was detected using rabbit anti-CRM1 Antigen Affinity purified polyclonal antibody (Catalog # ABO12651) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
  • IHC - Anti-CRM1 Picoband Antibody ABO12651
    CRM1 was detected in paraffin-embedded sections of mouse intestine tissues using rabbit anti- CRM1 Antigen Affinity purified polyclonal antibody (Catalog # ABO12651) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
  • IHC - Anti-CRM1 Picoband Antibody ABO12651
    CRM1 was detected in paraffin-embedded sections of rat testis tissues using rabbit anti- CRM1 Antigen Affinity purified polyclonal antibody (Catalog # ABO12651) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
  • IHC - Anti-CRM1 Picoband Antibody ABO12651
    CRM1 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- CRM1 Antigen Affinity purified polyclonal antibody (Catalog # ABO12651) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA2002).
  • IHC - Anti-CRM1 Picoband Antibody ABO12651
    Figure 5. IHC analysis of CRM1 using anti-CRM1 antibody (ABO12651).CRM1 was detected in frozen section of mouse intestine tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRM1 Antibody (ABO12651) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
  • IHC - Anti-CRM1 Picoband Antibody ABO12651
    Figure 6. IHC analysis of CRM1 using anti-CRM1 antibody (ABO12651).CRM1 was detected in frozen section of rat intestine tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRM1 Antibody (ABO12651) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
  • IHC - Anti-CRM1 Picoband Antibody ABO12651
    Figure 7. IHC analysis of CRM1 using anti-CRM1 antibody (ABO12651).CRM1 was detected in frozen section of rat testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRM1 Antibody (ABO12651) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F
Primary Accession O14980
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Exportin-1(XPO1) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 7514
Other Names Exportin-1, Exp1, Chromosome region maintenance 1 protein homolog, XPO1, CRM1
Calculated MW 123386 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, By Heat
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml

Western blot, 0.1-0.5 µg/ml
Subcellular Localization Cytoplasm. Nucleus, nucleoplasm. Nucleus, Cajal body. Nucleus, nucleolus. Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.
Tissue Specificity Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney. .
Protein Name Exportin-1
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human CRM1 recombinant protein (Position: N966-D1071). Human CRM1 shares 93.4% and 91.5% amino acid (aa) sequence identity with mouse and rat CRM1, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name XPO1
Synonyms CRM1
Function Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap.
Cellular Location Cytoplasm. Nucleus, nucleoplasm. Nucleus, Cajal body. Nucleus, nucleolus. Note=Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm
Tissue Location Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney
Research Areas
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Background

Exportin 1 (XPO1), also known as chromosomal maintenance 1 (CRM1), is an eukaryotic protein that mapped to human chromosome 2p16 by fluorescence in situ hybridization. This protein mediates leucine-rich nuclear export signal (NES)-dependent protein transport. It specifically inhibits the nuclear export of Rev and U snRNAs. Additionally, this protein is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. It also regulates NFAT and AP-1.

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$ 280.00
Cat# ABO12651
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