|Application ||WB, E|
|Description||Rabbit IgG polyclonal antibody for Kit ligand/Mast cell growth factor(KITLG) detection. Tested with WB, ELISA in Human.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Kit ligand, Mast cell growth factor, MGF, Stem cell factor, SCF, c-Kit ligand, Soluble KIT ligand, sKITLG, KITLG, MGF, SCF|
|Calculated MW||30899 MW KDa|
|Application Details||Western blot, 0.1-0.5 µg/ml, Human, -|
ELISA , 0.1-0.5 µg/ml, Human
|Subcellular Localization||Isoform 1: Cell membrane; Single-pass type I membrane protein.|
|Protein Name||Kit ligand|
|Contents||Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify Carrier Free" or "No BSA" in your order note. "|
|Immunogen||E. coli-derived human SCF recombinant protein(Position: E26-A190).|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the SCF family.|
|Function||Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.|
|Cellular Location||Isoform 1: Cell membrane; Single-pass type I membrane protein Soluble KIT ligand: Secreted.|
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Provided below are standard protocols that you may find useful for product applications.
Stem Cell Factor(also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor(CD117). The SCF gene is mapped to 12q21.32. SCF can exist both as a transmembrane protein and a soluble protein. This cytokine plays an important role in hematopoiesis(formation of blood cells), spermatogenesis, and melanogenesis. SCF may be used along with other cytokines to culture HSCs and hematopoietic progenitors. The expansion of these cells ex-vivo(outside the body) would allow advances in bone-marrow transplantation, in which HSCs are transferred to a patient to re-establish blood formation. One of the problems of injecting SCF for therapeutic purposes is that SCF activates mast cells. The injection of SCF has been shown to cause allergic-like symptoms and the proliferation of mast cells and melanocytes.
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