|Application ||WB, IHC-P, E|
|Description||Rabbit IgG polyclonal antibody for Serotransferrin(TF) detection. Tested with WB, IHC-P, ELISA in Human.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin, TF|
|Calculated MW||77064 MW KDa|
|Application Details||ELISA , 0.1-0.5 µg/ml, Human, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat
Western blot, 0.1-0.5 µg/ml, Human
|Tissue Specificity||Expressed by the liver and secreted in plasma.|
|Contents||Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||Oryza sativa-derived human Transferrin recombinant protein(Position: V20-P698).|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the transferrin family.|
|Function||Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.|
|Tissue Location||Expressed by the liver and secreted in plasma.|
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Provided below are standard protocols that you may find useful for product applications.
Transferrins are iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. In humans, it is encoded by the TF gene. In humans, transferrin consists of a polypeptide chain containing 679 amino acids. The protein is composed of alpha helices and beta sheets to form two domains. The N- and C- terminal sequences are represented by globular lobes and between the two lobes is an iron-binding site. Transferrin is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1%(4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover(25 mg/24 h). Transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of transferrin for Fe(III) is extremely high(1023 Mâˆ’1 at pH 7.4) but decreases progressively with decreasing pH below neutrality.
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