|Application ||IHC-P, IHC-F, FC, ICC|
|Description||Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 8(TNFSF8) detection. Tested with IHC-P, IHC-F, ICC, FCM in Human.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Tumor necrosis factor ligand superfamily member 8, CD30 ligand, CD30-L, CD153, TNFSF8, CD30L, CD30LG|
|Calculated MW||26017 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, By Heat|
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml
Immunocytochemistry, 0.5-1 µg/ml
Flow Cytometry, 1-3Î¼g/1x106cells
|Subcellular Localization||Membrane; Single-pass type II membrane protein.|
|Protein Name||Tumor necrosis factor ligand superfamily member 8|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E.coli-derived human CD153 recombinant protein (Position: Q63-D234). Human CD153 shares 75% amino acid (aa) sequence identity with mouse CD153.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins.|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Function||Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.|
|Cellular Location||Membrane; Single-pass type II membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
CD153 is a ligand for CD30. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene.
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