|Reactivity||Human, Mouse, Rat|
|Calculated MW||30602 Da|
|Application & Usage||Western blot analysis (5-10 µg/ml) and immunoprecipitation. The antibody detects a 12.0 kDa protein corresponding to the apparent molecular weight of cytochrome c.|
|Other Names||Cytochrome c-type heme lyase, CCHL, 188.8.131.52, Holocytochrome c-type synthase, HCCS, CCHL|
|Formulation||100 µg (0.2 mg/ml) purified IgG in PBS containing 50% glycerol, 1% BSA and 0.02% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Holocytochrome c Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Links covalently the heme group to the apoprotein of cytochrome c.|
|Cellular Location||Mitochondrion inner membrane. Membrane; Lipid-anchor|
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Provided below are standard protocols that you may find useful for product applications.
The precursor of cytochrome c, apocytochrome c, is synthesized in the cytoplasm. Upon translocation into mitochondria, cytochrome c acquires a heme moiety required for functionality in the mitochondrial respiration chain. The heme-bound form of cytochrome c is called holocytochrome c. When released into the cytosol, cytochrome c can activate caspases responsible for apoptosis.
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