|Reactivity||Human, Mouse, Rat|
|Calculated MW||53684 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml).However, the optimal conditions should be determined individually. 3T3 cell lysate can be used as a positive control. The antibody detects 60 kDa Fox04 from samples of human, mouse, and rat origins.|
|Other Names||FOXO4/FAX , AFX , AFX1 , MLLT7 , AFX , FoxO4 , AFX|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-AFX polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||AFX Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||AFX, AFX1, MLLT7|
|Function||Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.|
|Cellular Location||Cytoplasm. Nucleus. Note=When phosphorylated, translocated from nucleus to cytoplasm Dephosphorylation triggers nuclear translocation Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm|
|Tissue Location||Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
Fox04 is a member of the Forkhead family of transcription factors which are involved in tumorigenesis and other pathological processes. Fox04 has been shown to mediate signaling via a pathway involving IGFR1, PI3K, and Akt.
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