|Reactivity||Human, Mouse, Rat|
|Calculated MW||46844 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~47 kDa Trek 1 of human, mouse, and rat origins. Reactivity to other species has not been tested.|
|Other Names||K2p2.1; KCNK2; MGC126742; MGC126744; TPKC1; TREK; TREK-1; TREK1; hTREK-1c; hTREK-1e|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Trek-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Trek1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ion channel that contributes to passive transmembrane potassium transport. Reversibly converts between a voltage- insensitive potassium leak channel and a voltage-dependent outward rectifying potassium channel in a phosphorylation-dependent manner. In astrocytes, forms mostly heterodimeric potassium channels with KCNK1, with only a minor proportion of functional channels containing homodimeric KCNK2 (PubMed:24496152). In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1 (PubMed:24496152).|
|Cellular Location||Isoform 1: Cell membrane; Multi- pass membrane protein. Note=Location at the cell membrane requires interaction with KCNK1. Is not detected at the cell membrane when KCNK1 is absent.|
|Tissue Location||Detected in hippocampus astrocytes (at protein level) (PubMed:24496152). High expression in brain and lung. Also detected in kidney, heart and skeletal muscle. Not detected in liver. In the brain, highest expression in olfactory bulb, hippocampus and cerebellum.|
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Trek-1 and Trek-2 belong to the tandem-pore K+ channel family that has two pore-forming domains and four transmembrane segments. Trek-1 is expressed thro µghout the central nervous system whereas Trek-2 is found mostly in the cerebellum, spleen and testis. Trek-1 is activated by arachidonic acid and polyunsaturated fatty acids.
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